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In vitro antifungal efficacy of selected essential oils in controlling fungi associated with the stem-end rot disease of mango (cv. Karutha Colomban) fruits and characterisation of antifungal components

Authors:

TD Kodituwakku,

University of Kelaniya, LK
About TD
Department of Plant and Molecular Biology
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GCM Ekanayake,

University of Kelaniya, LK
About GCM
Department of Plant and Molecular Biology
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KP Abeywickrama ,

University of Kelaniya, LK
About KP
Department of Plant and Molecular Biology
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R Jayakody

The Open University of Sri Lanka, LK
About R
Department of Botany, Faculty of Natural Sciences,
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Abstract

Karutha Colomban is one of the most delicious mango varieties popular among Sri Lankan consumers. A significant postharvest loss of mango takes place in every season due to diseases including stem-end rot (SER), which is caused by a group of endophytic fungal pathogens. In this research, in vitro antifungal efficacy of different concentrations of essential oils of basil, clove, and cinnamon were evaluated for their ability to control SER causing fungal pathogens of mango (cv. Karutha Colomban) as bio-safe alternatives to conventional fungicides by conducting liquid and disc volatilisation bioassays. Major bioactive compounds of the selected essential oils were identified by gas chromatography-mass spectroscopy (GC-MS). Basil and cinnamon bark oils (0.20–0.30 μL/mL) in liquid bioassay showed high efficacy against Lasiodiplodia theobromae, while basil and cinnamon leaf oils (0.40–0.60 μL/mL) successfully inhibited Pestalotiopsis sp. Cinnamon bark oil (0.60 μL/mL) was identified as the most effective oil against Phomopsis sp. According to disc volatilisation bioassay, vapour of cinnamon oils (0.20–0.40 μL/mL) were the most effective in controlling Lasiodiplodia theobromae. Pestalotiopsis sp. was efficiently controlled by clove and cinnamon bark oil (0.20–0.60 μL/mL) vapour. In vapour phase, clove and cinnamon oils (0.40 μL/mL) were the most effective against Phomopsis sp. According to GC-MS characterisation, methyl chavicol was the most abundant antifungal component in basil oil while it was (E)-cinnamaldehyde in cinnamon bark oil. Moreover, eugenol displayed the highest abundance in clove and cinnamon leaf oils. Based on in vitro studies, it could be concluded that cinnamon bark oil in liquid and vapour phases demonstrated a higher antifungal efficacy among the tested essential oils in controlling fungal pathogens causing SER of mango. 

How to Cite: Kodituwakku, T., Ekanayake, G., Abeywickrama, K. and Jayakody, R., 2020. In vitro antifungal efficacy of selected essential oils in controlling fungi associated with the stem-end rot disease of mango (cv. Karutha Colomban) fruits and characterisation of antifungal components. Journal of the National Science Foundation of Sri Lanka, 48(2).
Published on 21 Jul 2020.
Peer Reviewed

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