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Improved protocol for efficient regeneration of coconut (Cocos nucifera L.) anther derived embryos

Authors:

H.D.D. Bandupriya ,

University of Colombo, LK
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P. Waidyarathne

Coconut Research Institute, LK
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Abstract

The occurrence of severe shoot necrosis and other constraints, such as low frequency of embryo induction and poor regeneration of plants, restricts the use of coconut androgenesis in practice. Fine-tuning of the protocol by addressing the above constraints was carried out with the intention of scaling up haploid plant production. Out of the carbon types, sucrose and maltose when added in concentrations of  90.0 gL-1 and 120.0 gL-1 showed significantly higher (p < 0.05) embryo (44.0 % and 36.0 % respectively) production. Out of the concentrations used in the study, 20.0 μM 6-benzylaminopurine BAP showed significantly (p = 0.001) higher shoot generation (47.6 %) as well as significantly (p = 0.006) longer shoot production (31.7 %) within the study period. The effect of CaCl2 on the reduction of shoot necrosis was also tested. CaCl2 showed a significant (p = 0.001) effect on reducing shoot necrosis. The lowest occurrence of shoot necrosis (25.0 %) was observed in 4.0 mM CaCl2 treatment. Continuous sub-culturing of shoots with initial signs of shoot necrosis, to elevated CaCl2 levels until the rooting stage, facilitated the recovery. The transfer of shoots frequently in to a fresh medium was not beneficial for the suppression of necrosis. Shoots maintained in the medium enriched with 4.0 mM CaCl2 were transferred for acclimatisation, and this is the first report of transferring haploid coconut plants to acclimatisation conditions. The rooted shoots produced through the optimised protocol were acclimatised successfully. The prevention of shoot loss due to shoot necrosis will be beneficial for further refinement of the coconut anther culture protocol. 

How to Cite: Bandupriya, H.D.D. and Waidyarathne, P., 2021. Improved protocol for efficient regeneration of coconut (Cocos nucifera L.) anther derived embryos. Journal of the National Science Foundation of Sri Lanka, 49(3), pp.383–391.
Published on 30 Nov 2021.
Peer Reviewed

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