Research Articles
Production and characterization of β-galactosidase from the fungus Thielaviopsis ethacetica Went.
Authors:
D.M.S. Dissanayaka,
Wayamba University of Sri Lanka, LK
About D.M.S.
Department of Biotechnology
S.N.T. De Silva ,
Wayamba University of Sri Lanka, LK
About S.N.T.
Department of Material and NanoScience Technology
D.P.S.T.G. Attanayaka,
Wayamba University of Sri Lanka, LK
About D.P.S.T.G.
Department of Biotechnology
E.M.M. Vimukthi
Wayamba University of Sri Lanka, LK
About E.M.M.
Department of Biotechnology
Abstract
β-galactosidase (EC 3.2.1.23) is an industrially important enzyme. It can catalyse two different types of reactions, namely lactose hydrolysis and transgalactosylation. β-galactosidase enzyme production by Thielaviopsis ethacetica, was investigated and the enzyme was partially purified and characterized in the present study. Qualitative assay for the pre β-galactosidase activity indicated the formation of water insoluble blue-green colour, dichloro-dibromo-indigo zone towards the edges of the fungal colony after a 72-hour incubation period. Quantitative assay recorded a maximum enzyme activity of 0.599 U/mL at 72 hours. The enzyme was partially purified from the crude enzyme using ammonium sulphate precipitation and dialysis. Fraction 40-60 % (fraction 2) had the highest specific enzymes activity of 1.65 U/mg with 8.68 purification fold and 28.3 % recovery. SDS-PAGE analysis of fraction 2 resulted three separate protein bands above 40 kDa. The protein band corresponding to 50 kDa molecular weight was confirmed as β-galactosidase. The optimum temperature and pH for the enzyme was at 60 ̊ C and 7.0, respectively. The present study reveals the fungal strain Thielaviopsis ethacetica with β-galactosidase activity. To our knowledge this is the first report about β-galactosidase activity of the strain T. ethacetica. This investigation suggests
How to Cite:
Dissanayaka, D.M.S., De Silva, S.N.T., Attanayaka, D.P.S.T.G. and Vimukthi, E.M.M., 2021. Production and characterization of β-galactosidase from the fungus Thielaviopsis ethacetica Went.. Journal of the National Science Foundation of Sri Lanka, 49(4), pp.563–571. DOI: http://doi.org/10.4038/jnsfsr.v49i4.10335
Published on
31 Dec 2021.
Peer Reviewed
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