Research Articles
A PEG 4000-degrading and hexavalent molybdenum-reducing Pseudomonas putida strain Egypt-15
Authors:
M Abd. AbdEl-Mongy,
Sadat City University, EG
About M Abd.
Microbial Biotechnology Department, Genetic Engineering and Biotechnology Institute
SA Aqlima,
Universiti Putra Malaysia, MY
About SA
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences
MS Shukor,
MY
About MS
Snoc International Sdn Bhd
S Hussein,
Malaysian Nuclear Agency, MY
About S
Agrotechnology and Bioscience Division
APK Ling ,
International Medical University, MY
About APK
Division of Applied Biomedical Sciences & Biotechnology, School of Health Sciences
MY Shukor
Universiti Putra Malaysia, MY
About MY
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences
Abstract
The objectives of this work were to isolate and characterise a heavy metal-reducing bacterium with the capability to degrade another xenobiotic; an organic pollutant. Six molybdenum-reducing bacteria from soil that can reduce sodium molybdate into the colloidal molybdenum blue (Mo-blue) were isolated. One of these isolates identified as Pseudomonas putida strain Egypt-15 was capable of growing on PEG 4000. The optimal conditions for Mo-blue production were 34 °C, pH 6.5, 20 mM molybdate, and glucose as the electron donor. The optimum concentration supporting the growth on PEG 4000 was between 600 and 800 mgL-1. PEG degradation showed a lag period of about two days and 75 % degradation of PEG 4000 was achieved on day six at 800 mgL-1. Growth on PEG 4000 at 800 mgL-1 modelled according to the modified Gompertz model gave a maximum specific growth rate of 2.216 d-1 and a lag period of 1.45 days. Growth on PEG was optimum at 30 °C and pH 7.5. The dual ability of this bacterium to detoxify molybdenum and degrade PEG is novel and will be very useful for bioremediation.
How to Cite:
AbdEl-Mongy, M.A., Aqlima, S., Shukor, M., Hussein, S., Ling, A. and Shukor, M., 2018. A PEG 4000-degrading and hexavalent molybdenum-reducing Pseudomonas putida strain Egypt-15. Journal of the National Science Foundation of Sri Lanka, 46(3), pp.431–442. DOI: http://doi.org/10.4038/jnsfsr.v46i3.8495
Published on
30 Sep 2018.
Peer Reviewed
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