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A PEG 4000-degrading and hexavalent molybdenum-reducing Pseudomonas putida strain Egypt-15

Authors:

M Abd. AbdEl-Mongy,

Sadat City University, EG
About M Abd.
Microbial Biotechnology Department, Genetic Engineering and Biotechnology Institute
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SA Aqlima,

Universiti Putra Malaysia, MY
About SA
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences
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MS Shukor,

MY
About MS

Snoc International Sdn Bhd

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S Hussein,

Malaysian Nuclear Agency, MY
About S
Agrotechnology and Bioscience Division
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APK Ling ,

International Medical University, MY
About APK
Division of Applied Biomedical Sciences & Biotechnology, School of Health Sciences
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MY Shukor

Universiti Putra Malaysia, MY
About MY
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences
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Abstract

The objectives of this work were to isolate and characterise a heavy metal-reducing bacterium with the capability to degrade another xenobiotic; an organic pollutant. Six molybdenum-reducing bacteria from soil that can reduce sodium molybdate into the colloidal molybdenum blue (Mo-blue) were isolated. One of these isolates identified as Pseudomonas putida strain Egypt-15 was capable of growing on PEG 4000. The optimal conditions for Mo-blue production were 34 °C, pH 6.5, 20 mM molybdate, and glucose as the electron donor. The optimum concentration supporting the growth on PEG 4000 was between 600 and 800 mgL-1. PEG degradation showed a lag period of about two days and 75 % degradation of PEG 4000 was achieved on day six at 800 mgL-1. Growth on PEG 4000 at 800 mgL-1 modelled according to the modified Gompertz model gave a maximum specific growth rate of 2.216 d-1 and a lag period of 1.45 days. Growth on PEG was optimum at 30 °C and pH 7.5. The dual ability of this bacterium to detoxify molybdenum and degrade PEG is novel and will be very useful for bioremediation.

How to Cite: AbdEl-Mongy, M.A., Aqlima, S., Shukor, M., Hussein, S., Ling, A. and Shukor, M., 2018. A PEG 4000-degrading and hexavalent molybdenum-reducing Pseudomonas putida strain Egypt-15. Journal of the National Science Foundation of Sri Lanka, 46(3), pp.431–442. DOI: http://doi.org/10.4038/jnsfsr.v46i3.8495
Published on 30 Sep 2018.
Peer Reviewed

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