Start Submission Become a Reviewer

Reading: Optimising a solvent system for lipid extraction from cyanobacterium Microcystis spp.: futur...

Download

A- A+
Alt. Display

Research Articles

Optimising a solvent system for lipid extraction from cyanobacterium Microcystis spp.: future perspective for biodiesel production

Authors:

D.A.T Madusanka,

University of Sri Jayewardenepura, LK
About D.A.T
Centre for Water Quality and Algae research, Department of Zoology, Faculty of Applied Sciences
X close

Pathmalal M. Manage

University of Sri Jayewardenepura, LK
About Pathmalal
Centre for Water Quality and Algae research, Department of Zoology, Faculty of Applied Sciences
X close

Abstract

ue to unsustainability of petro derived fuels, the world is now concerned about alternative, sustainable and eco-friendly energy production. As a successful alternative energy, biodiesel plays a major role to replace petrodiesel. Cyanobacterial lipids have great potential for biodiesel production due to their rapid growth rate and renewability. The production of biodiesel from biomasses depends on upstream (lipid extraction) and downstream (trans-esterification) processes. Lipid extraction should be effective and energy efficient to reduce the production cost of commercial biodiesel production. Therefore, the present study was experimented to optimise the lipid extraction solvent system from cyanobacteria.

Cyanobacteria samples were collected from Beira Lake, Colombo where, Microcystis spp. are dominant (> 96 %). Solvent systems n-hexane, chloroform: methanol (2:1), n-hexane: ethanol (3:2), chloroform: n-hexane (1:1), dichloromethane: methanol (1:1), n-hexane: isopropanol (2:3) and diethyl ether were used for lipid extraction and subjected to optimisation for the lipid extraction method from the cyanobacterium Microcystis spp. Extracted lipid yield (g/1 g of dried biomass) for each solvent system was 1.11 wt. %, 1.61 wt. %, 1.71 wt. %, 2.15 wt. %, 3.41 wt. %, 4.33 wt. % and 1.95 wt. %, respectively where fatty acid methyl ester (FAME) compositions found to be 80.13 %, 78.27 %, 76.39 %, 29.01 %, 85.72 %, 92.39 % and 80.09 % in each system. The gas chromatographic-mass spectrometry analysis of the products revealed that the most abundant fatty acid types of Microcystis spp. were palmitic acid (C16:0) and its derivative such as 14-methyl pentadecanoic acid. The highest lipid yield (4.33 wt. %) and fatty acid methyl ester (92.39 %) composition were recorded from n-hexane: isopropanol solvent system.

How to Cite: Madusanka, D.A.T. and M. Manage, P., 2018. Optimising a solvent system for lipid extraction from cyanobacterium Microcystis spp.: future perspective for biodiesel production. Journal of the National Science Foundation of Sri Lanka, 46(2), pp.217–225. DOI: http://doi.org/10.4038/jnsfsr.v46i2.8422
Published on 30 Jun 2018.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus