Research Articles
In vitro clonal propagation of Coscinium fenestratum (Gertn.) Colebr. (Weniwel) through nodal explants
Authors:
PS Warakagoda ,
LK
About PS
Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya.
S Subasinghe,
LK
About S
Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya.
MTK Gunasekare
LK
About MTK
Coordinating Secretariat for Science, Technology and Innovation, 3rd Floor, Standard Chartered Building, Janadhipathi Mawatha, Colombo 01.
Abstract
An in vitro clonal propagation protocol for Coscinium fenestratum was developed using shoot explants detached from 1 − 2 year old vines maintained under plant house conditions, by successfully surface sterilising with 0.2 % solution of mercuric chloride for 30 minutes followed by two successive washings with sterilised distilled water. McCowns woody plant medium (WPM) incorporated with 1.0 mgL-1 polyvinylpyrrolidone to minimise browning, was the best medium for establishment of nodal cuttings. Mature double nodal cuttings resulted in the highest shoot proliferation rate (3.90 shoots/explant) when cultured on WPM medium supplemented with 2.0 mgL-1 6-benzylaminopurine, 1.0 mgL-1 thidiazuron and 0.4 mgL-1 2,4-dichlorophenoxyacetic acid. Shoots were separated and transferred to WPM medium devoid of plant growth regulators for regeneration into plantlets. The plantlets were successfully acclimatised on coir dust: sand (1:1) potting media with over 60 % survival rate. The results proved that the protocol developed is effective for clonal propagation of C. fenestratum.
How to Cite:
Warakagoda, P., Subasinghe, S. and Gunasekare, M., 2017. In vitro clonal propagation of Coscinium fenestratum (Gertn.) Colebr. (Weniwel) through nodal explants. Journal of the National Science Foundation of Sri Lanka, 45(2), pp.133–141. DOI: http://doi.org/10.4038/jnsfsr.v45i2.8179
Published on
29 Jun 2017.
Peer Reviewed
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