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In vitro clonal propagation of Coscinium fenestratum (Gertn.) Colebr. (Weniwel) through nodal explants

Authors:

PS Warakagoda ,

LK
About PS
Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya.
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S Subasinghe,

LK
About S
Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya.
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MTK Gunasekare

LK
About MTK
Coordinating Secretariat for Science, Technology and Innovation, 3rd Floor, Standard Chartered Building, Janadhipathi Mawatha, Colombo 01.    
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Abstract

An in vitro clonal propagation protocol for Coscinium  fenestratum was developed using shoot explants detached from  1 − 2 year old vines maintained under plant house conditions, by  successfully surface sterilising with 0.2 % solution of mercuric  chloride for 30 minutes followed by two successive washings  with sterilised distilled water. McCowns woody plant medium (WPM) incorporated with 1.0 mgL-1 polyvinylpyrrolidone to minimise browning, was the best medium for establishment of nodal cuttings.  Mature double nodal cuttings resulted in the highest shoot  proliferation rate (3.90 shoots/explant) when cultured on WPM  medium supplemented with 2.0 mgL-1 6-benzylaminopurine,  1.0 mgL-1 thidiazuron and 0.4 mgL-1 2,4-dichlorophenoxyacetic  acid. Shoots were separated and transferred to WPM medium devoid of plant growth regulators for regeneration into plantlets. The plantlets were successfully acclimatised on coir dust: sand (1:1) potting media with over 60 % survival rate. The results proved that the protocol developed is effective for clonal propagation of C. fenestratum.  

How to Cite: Warakagoda, P., Subasinghe, S. & Gunasekare, M., (2017). In vitro clonal propagation of Coscinium fenestratum (Gertn.) Colebr. (Weniwel) through nodal explants. Journal of the National Science Foundation of Sri Lanka. 45(2), pp.133–141. DOI: http://doi.org/10.4038/jnsfsr.v45i2.8179
Published on 29 Jun 2017.
Peer Reviewed

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