Short Communications
Purification of xylanase produced by Bacillus pumilus
Authors:
Ranganathan Kapilan,
LK
About Ranganathan
Department of Botany, Faculty of Science, University of Jaffna, Jaffna.
Vasanthy Arasaratnam
LK
About Vasanthy
Department of Biochemistry, Faculty of Medicine, University of Jaffna, Adiyapatham Road, Kokuvil West, Kokuvil.
Abstract
This study was aimed at purifying xylanase produced by Bacillus pumilus. The spent medium contained 27.9 UmL-1 xylanase activity and 1.5 mgmL-1 protein. The highest specific activity (33.7 Umg-1 protein) was achieved with 50 % (NH4)2SO4 saturation and the xylanase recovery was 94.8 %. The dialyzed and DEAE-Sepharose purified enzyme showed 6.7-fold increase in specific activity with a yield of 84.2 %. Molecular weight of the purified xylanase was 55.4 KDa. Thus B. pumilus xylanase can be purified by precipitating with 50 % (NH4)2SO4 saturation and DEAE-Sepharose ion exchange chromatography.
DOI: http://dx.doi.org/10.4038/jnsfsr.v42i4.7736
J.Natn.Sci.Foundation Sri Lanka 2014 42 (4):365-368
How to Cite:
Kapilan, R. and Arasaratnam, V., 2014. Purification of xylanase produced by Bacillus pumilus. Journal of the National Science Foundation of Sri Lanka, 42(4), pp.365–368. DOI: http://doi.org/10.4038/jnsfsr.v42i4.7736
Published on
03 Dec 2014.
Peer Reviewed
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