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Purification of xylanase produced by Bacillus pumilus

Authors:

Ranganathan Kapilan,

LK
About Ranganathan
Department of Botany, Faculty of Science, University of Jaffna, Jaffna.
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Vasanthy Arasaratnam

LK
About Vasanthy
Department of Biochemistry, Faculty of Medicine, University of Jaffna, Adiyapatham Road, Kokuvil West, Kokuvil.
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Abstract

This study was aimed at purifying xylanase produced by Bacillus pumilus. The spent medium contained 27.9 UmL-1 xylanase activity and 1.5 mgmL-1 protein. The highest specific activity (33.7 Umg-1 protein) was achieved with 50 % (NH4)2SO4 saturation and the xylanase recovery was 94.8 %. The dialyzed and DEAE-Sepharose purified enzyme showed 6.7-fold increase in specific activity with a yield of 84.2 %. Molecular weight of the purified xylanase was 55.4 KDa. Thus B. pumilus xylanase can be purified by precipitating with 50 % (NH4)2SO4 saturation and DEAE-Sepharose ion exchange chromatography.

DOI: http://dx.doi.org/10.4038/jnsfsr.v42i4.7736

J.Natn.Sci.Foundation Sri Lanka 2014 42 (4):365-368

How to Cite: Kapilan, R. and Arasaratnam, V., 2014. Purification of xylanase produced by Bacillus pumilus. Journal of the National Science Foundation of Sri Lanka, 42(4), pp.365–368. DOI: http://doi.org/10.4038/jnsfsr.v42i4.7736
Published on 03 Dec 2014.
Peer Reviewed

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