Start Submission Become a Reviewer

Reading: Oligomeric proanthocyanidin fractions from fresh tea leaves and their antibacterial activity...

Download

A- A+
Alt. Display

Research Articles

Oligomeric proanthocyanidin fractions from fresh tea leaves and their antibacterial activity against Staphylococcus aureus

Authors:

N Savitri Kumar ,

LK
About N Savitri
Institute of Fundamental Studies, Hantana Road, Kandy.
X close

BM Ratnayake Bandara,

LK
About BM Ratnayake
Department of Chemistry, Faculty of Science, University of Peradeniya, Peradeniya.
X close

Sujeewa K Hettihewa,

LK
About Sujeewa K
Department of Chemistry, Faculty of Science, University of Peradeniya, Peradeniya.
X close

Gehan J Panagoda

LK
About Gehan J
Division of Microbiology, Faculty of Dental Sciences, University of Peradeniya, Peradeniya.
X close

Abstract

Purification of the proanthocyanidin (PA) extract of fresh tea [Camellia sinensis (L.) Kuntze] leaves by Sephadex LH-20 column chromatography followed by high-speed counter-current chromatography (HSCCC) using the two phase solvent system hexane-ethyl acetate-methanol-water (1:5:1:5) furnished three PA fractions PA1- PA3. The electro-spray ionization mass spectrometry (ESI-MS) of the PA fractions indicated the presence of oligomeric PAs in all three PA fractions. Antibacterial activity against Staphylococcus aureus and their minimum inhibitory concentration (MIC) values were determined using the agar well diffusion assay and the agar dilution method, respectively. The antibacterial activity was observed for the selected PA fractions with MIC values in the range 512 − 1024 μg/mL against methicillin-resistant S. aureus (MRSA, 12 strains), methicillin-susceptible S. aureus (MSSA, 1 strain) and two standard strains of S. aureus, NCTC 6571 and ATCC 25923S. This is the first report of antibacterial activity displayed by tetrameric A-type PAs in the extracts of fresh tea leaves.

DOI: http://dx.doi.org/10.4038/jnsfsr.v42i3.7397

J.Natn.Sci.Foundation Sri Lanka 2014 42 (3): 241-246

How to Cite: Kumar, N.S., Bandara, B.R., Hettihewa, S.K. and Panagoda, G.J., 2014. Oligomeric proanthocyanidin fractions from fresh tea leaves and their antibacterial activity against Staphylococcus aureus. Journal of the National Science Foundation of Sri Lanka, 42(3), pp.241–246. DOI: http://doi.org/10.4038/jnsfsr.v42i3.7397
Published on 10 Sep 2014.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus