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Enhanced production of ethanol by high gravity glucose fermentation at temperatures above 40 oC by Saccharomyces cerevisiae S1 using a soya flour supplemented medium

Authors:

Sandrasegarampillai Balakumar,

LK
About Sandrasegarampillai
Department of Biochemistry, Faculty of Medicine, University of Jaffna, Kokuvil.
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Vasanthy Arasaratnam

LK
About Vasanthy
Department of Biochemistry, Faculty of Medicine, University of Jaffna, Kokuvil.
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Abstract

A locally isolated strain of Saccharomyces cerevisiae S1 in peptone, yeast and mineral nutrient (PYN) medium with 100 gL-1 of glucose showed optimum growth at 36 oC and efficient ethanol production at 40 oC. To investigate the effect of soya flour supplementation on ethanol fermentation, PYN medium containing different concentrations of glucose (50 to 400 gL-1) with and without soya flour (26.8 gl-1) were used at 40 ºC for 48 h. The above experiment was repeated at different temperatures (40, 43 and 45 ºC) for 48 h to find the effect of soya flour supplementation on ethanol production at higher temperatures and sugar levels. The highest ethanol production efficiency (96.5 %) and the highest ethanol yield (149.3 gL-1) with efficient glucose utilization (100 %) was observed in soya flour supplemented medium with a sugar concentration of 300 gL-1 at 40 ºC. Further, it was observed that at higher temperatures (43 and 45 ºC) and higher sugar concentrations, soya flour supplemented media performed well. With 300 gL-1 glucose at 43 and 45 oC the amounts of ethanol produced in soya flour supplemented media was 80 and 30 gl-1, respectively while in unsupplemented media it was only 28 and 18 gL-1, respectively. In glucose (400 gl-1) – PYN medium supplemented with soya flour, 78 (48 h) and 25 gl-1 (48 h) ethanol was produced at 43 and 45°C, respectively. The study revealed that supplementation of media with soya flour has not only improved the glucose fermenting capacity but has also increased the efficiency of ethanol production and the ethanol production rate at higher temperatures and high sugar levels.

DOI: http://dx.doi.org/10.4038/jnsfsr.v42i2.6987

J.Natn.Sci.Foundation Sri Lanka 2014 42 (2): 111-117

 

How to Cite: Balakumar, S. and Arasaratnam, V., 2014. Enhanced production of ethanol by high gravity glucose fermentation at temperatures above 40 oC by Saccharomyces cerevisiae S1 using a soya flour supplemented medium. Journal of the National Science Foundation of Sri Lanka, 42(2), pp.111–117. DOI: http://doi.org/10.4038/jnsfsr.v42i2.6987
Published on 09 Jun 2014.
Peer Reviewed

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