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Bioactivity evaluation of organic solvent extractions of Ganoderma lucidum: a Sri Lankan basidiomycete

Authors:

Kalpa W Samarakoon,

Faculty of Science, University of Colombo, Colombo 03, LK
About Kalpa W
Department of Chemistry
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Ji-Hyeok Lee,

School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea, KR
About Ji-Hyeok
Department of Marine Life Science
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E Dilip De Silva,

Faculty of Science, University of Colombo, Colombo 03, LK
About E Dilip
Department of Chemistry
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Eun-A Kim,

School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea, KR
About Eun-A
Department of Marine Life Science
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Ravi LC Wijesundara,

Faculty of Science, University of Colombo, Colombo 03, LK
About Ravi LC
Department of Plant Sciences
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HH Chaminda Lakmal,

Faculty of Science, University of Colombo, Colombo 03, LK
About HH Chaminda
Department of Chemistry
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You-Jin Jeon

School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, Republic of Korea, KR
About You-Jin
Department of Marine Life Science
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Abstract

In this study, bioactivities of organic solvent extracts derived from nine species of terrestrial fungi were evaluated. The methanol extract of Ganoderma lucidum was selected as a potent sample with antibacterial activity showing growth inhibitory effects against pathogenic bacterial strains. Solvent-solvent partition chromatography was used to seperate four fractions from the G. lucidum crude sample. Among the fractions, chloroform fraction was proved to comprise seven major compounds in HPLC analysis. The positive peak 4, at 27.0 min retention time on the UV detector at 290 and 365 nm, which corresponded to the negative peak at 680 nm on-line HPLC-ABTS+ chromatogram was identified as an antioxidant compound. Antioxidant activity against 2,2-diphenyl-1- picrylhydrazyl (DPPH) was tested using radical scavenging activity with the electron spin resonance spectrometer (ESR). The highest activity was shown by the chloroform fraction with a IC50 value 0.684 ± 0.31 mg mL-1. The inhibitory effect of nitric oxide (NO) production (%) was reported as the highest with the IC50 value 30.38 ± 0.03 μg mL-1 from chloroform fraction against lipopolysaccharide (LPS) induced RAW 264.7 cells in vitro. The methanol and ethyl acetate fractions also showed similar but lesser effects with IC50 values 33.09 ± 0.04 and 122.9 ± 0.82 μg mL-1, respectively. Cell viability (%) by the MTT assay confirmed that there was no cytotoxic effect when treated with G. lucidum extracts at all concentrations. These results demonstrate the potential of using chloroform extracts of G. lucidum in antibacterial, antioxidant and anti- inflammatory therapeutic applications.

J.Natn.Sci.Foundation Sri Lanka 2013 41 (3): 249-257

DOI: http://dx.doi.org/10.4038/jnsfsr.v41i3.6049

How to Cite: Samarakoon, K.W., Lee, J.-H., De Silva, E.D., Kim, E.-A., Wijesundara, R.L., Lakmal, H.C. and Jeon, Y.-J., 2013. Bioactivity evaluation of organic solvent extractions of Ganoderma lucidum: a Sri Lankan basidiomycete. Journal of the National Science Foundation of Sri Lanka, 41(3), pp.249–257. DOI: http://doi.org/10.4038/jnsfsr.v41i3.6049
Published on 15 Sep 2013.
Peer Reviewed

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