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Antixenosis and antibiosis effects of Oryza nivara accessions harbouring bph2 gene on brown planthopper [Nilaparvata lugens (Stal)]

Authors:

SAP Madurangi ,

LK
About SAP
Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya
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Disna Ratnasekera,

LK
About Disna
Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya
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SGJN Senanayake,

LK
About SGJN
Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya
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WLG Samarasinghe,

LK
About WLG
Rice Research and Development Institute, Bathalagoda, Ibbagamuwa
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PV Hemachandra

LK
About PV
Rice Research and Development Institute, Bathalagoda, Ibbagamuwa
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Abstract

Gene bph2 is one of the 21 brown planthopper (BPH), Nilaparvata lugens (Stal) resistant genes identified so far in several indica cultivars and wild relatives. The present study evaluated Oryza nivara accessions collected from different locations in Sri Lanka for bph2 and, antibiosis and antixenosis effects of O. nivara accessions harbouring bph2 gene on BPH. The five O. nivara accessions; WRAC 02, WRAC 04, WRAC 07, WRAC 21 and WRAC 25 amplified the resistant band (300 bp) indicating the presence of the bph2 gene in their genomes. The level of resistance observed in these accessions was high and showed negative effects on honeydew production and nymphal survival (antibiosis) and revealed antixenosis action against nymphs and oviposition. In addition, dynamic  response mechanisms of BPH against O. nivara accessions that possess bph2 was found, which may be indicative of the complex structure of genetics of resistance of these accessions. Substantial level of resistance similar to that in Ptb 33, observed in O. nivara accessions WRAC 02, WRAC 04 gives a clue to the possible existence of polygenic resistance as detected in Ptb 33. Results of this study highligh ted the possibility of the use of O. nivara accessions that possess bph2 gene in their genome as a potential source of resistance in pyramiding of genes for BPH resistance to avoid the genetic uniformity of BPH resistant rice varieties cultivated in Sri Lanka in the future.

DOI: http://dx.doi.org/10.4038/jnsfsr.v41i2.5709

J.Natn.Sci.Foundation Sri Lanka 2013 41(2):147-154

How to Cite: Madurangi, S., Ratnasekera, D., Senanayake, S., Samarasinghe, W. and Hemachandra, P., 2013. Antixenosis and antibiosis effects of Oryza nivara accessions harbouring bph2 gene on brown planthopper [Nilaparvata lugens (Stal)]. Journal of the National Science Foundation of Sri Lanka, 41(2), pp.147–154. DOI: http://doi.org/10.4038/jnsfsr.v41i2.5709
Published on 27 Jun 2013.
Peer Reviewed

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