Reading: Evaluation of species limits of Hortonia by DNA barcoding

Download

A- A+
dyslexia friendly

Short Communications

Evaluation of species limits of Hortonia by DNA barcoding

Authors:

Sanath Rajapakse ,

Department of Molecular Biology and Biotechnology, Faculty of Science, University of Peradeniya, Peradeniya., LK
X close

Prabasheeni Iddamalgoda,

Department of Chemistry, Faculty of Science, University of Peradeniya, Peradeniya., LK
X close

Rukmal Ratnayake,

Department of Chemistry, Faculty of Science, University of Peradeniya, Peradeniya., LK
X close

D.S.A. Wijesundara,

Department of National Botanic Gardens, P.O. Box 14, Peradeniya., LK
X close

B.M. Ratnayake Bandara,

Department of Chemistry, Faculty of Science, University of Peradeniya, Peradeniya., LK
X close

Veranja Karunaratne

Department of Chemistry, Faculty of Science, University of Peradeniya, Peradeniya., LK
X close

Abstract

A study was carried out to determine the species limits of the endemic genus Hortonia by DNA barcoding with a view to establishing conclusive molecular evidence regarding the speciation of Hortonia in Sri Lanka. Total DNA was extracted from all three species and the purity of the extracted DNA was confirmed. Internal transcribed spacer (ITS) region and the trnH-psbA region were first amplified by the polymerase chain reaction (PCR) with specific primers and ligated to a pBlueScript vector followed by plasmid purification. The purified plasmids containing the DNA of interest were subjected to sequencing. Sequence homology of ITS and trnH-psbA from all three species were compared using MacVector software. Between H. ovalifolia and H. floribunda, the ITS region showed a 2.37 % sequence divergence and the trnH-psbA region showed a 1.5 % sequence divergence. Between H. ovalifolia and H. angustifolia, the ITS region and trnH-psbA region showed a 3.36 % and 1.89 % sequence divergences, respectively. The percentage sequence divergence between ITS and trnH-psbA regions of H. floribunda and H. angustifolia were 3.36 % and 2.65 %, respectively. The high sequence divergence values clearly indicate that the genus Hortonia has three different species. Considering the percentage sequence divergence values, H. ovalifolia and H. floribunda are more closely related to each other than to H. angustifolia.

Doi: http://dx.doi.org/10.4038/jnsfsr.v40i4.5048

J.Natn.Sci.Foundation Sri Lanka 2012 40 (4): 345-349

How to Cite: Rajapakse, S. et al., (2012). Evaluation of species limits of Hortonia by DNA barcoding. Journal of the National Science Foundation of Sri Lanka. 40(4), pp.345–349. DOI: http://doi.org/10.4038/jnsfsr.v40i4.5048
Published on 19 Dec 2012.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus