Reading: Morphological aspects of coconut anther culture derived structures

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Morphological aspects of coconut anther culture derived structures

Authors:

P.I.P. Perera ,

Coconut Research Institute, LK
About P.I.P.
Tissue Culture Division, Coconut Research Institute, Bandirippuwa Estate, Lunuwila.
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D.M.D. Yakandawala,

University of Peradeniya, LK
About D.M.D.
Department of Botany, Faculty of Science, University of Peradeniya, Peradeniya
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J.L. Verdeil,

Institute for Research and Development (IRD), FR
About J.L.
Institute for Research and Development (IRD), UMR 1098 BEPC, IRD, BP 64501 - 911 Avenue Agropolis, 34394 Montpellier Cedex 1, France
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V. Hocher,

CIRAD, FR
About V.
CIRAD, TA40/02 Avenue Agropolis, 34398 Montpellier Cedex 5, France
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L. K. Weerakoon

University of Peradeniya, LK
About L. K.

Postgraduate Institute of Science, P.O. Box 25, University of Peradeniya, Peradeniya.

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Abstract

Anthers excised from male flowers of an adult coconut palm of Sri Lanka Tall cultivar were used to produce plants via androgenesis. Morphological aspects of the anther-derived structures and their plant regeneration pathways were studied. Under the culture conditions employed, both calli and embryo-mediated plant regeneration were observed. Two types of embryos with different germination patterns were identified-one with a germination point and the other type with a blunt haustorium without a prominent germination point which converted into germinated embryos. Three types of calli (embryogenic compact calli, embryogenic friable calli and non-embryogenic fast-growing calli) were also produced and the most commonly identified type was embryogenic compact calli that give rise to somatic embryos. Secondary somatic embryos were produced in both direct embryo and callus mediated embryogenesis. Normal plants with a single shoot were observed in low frequencies while weak plantlets with multiple shoot were observed in abundance. Under similar culture conditions, some plantlets showed vigorous growth whereas the majority had a slow growth rate.

Keywords: Androgenesis, anther culture, callus, coconut (Cocos nucifera L.), embryo, morphology

doi :10.4038/jnsfsr.v38i1.1727

J.Natn.Sci.Foundation Sri Lanka 2010 38 (1): 69-74

How to Cite: Perera, P.I.P. et al., (2010). Morphological aspects of coconut anther culture derived structures. Journal of the National Science Foundation of Sri Lanka. 38(1), pp.69–74. DOI: http://doi.org/10.4038/jnsfsr.v38i1.1727
Published on 31 Mar 2010.
Peer Reviewed

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