Start Submission Become a Reviewer

Reading: Somatic embryogenesis in papaya (Carica papaya L.) cv. Rathna

Download

A- A+
Alt. Display

Research Articles

Somatic embryogenesis in papaya (Carica papaya L.) cv. Rathna

Authors:

ARF Farzana ,

Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya., LK
X close

PGVN Palkadapala,

Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, LK
X close

KMMN Meddegoda,

Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, LK
X close

PK Samarajeewa,

Biotechnology Division, Plant Genetic Resource Centre, Gannoruwa, LK
X close

JP Eeswara

Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, LK
X close

Abstract

A protocol was developed for somatic embryogenesis from zygotic embryos, hypocotyl explants from in vitro seedlings and leaf explants from greenhouse grown plants of Carica papaya L. cv. Rathna. Seeds in which the sarcotesta membrane is removed, were germinated in conical flasks with sterilized water and maintained on an orbitral shaker, and within 2-4 week were used to excise hypocotyls explants. Zygotic embryos were isolated directly from sterilized seeds.

Embryogenic calli from zygotic embryos and hypocotyl explants were initiated successfully on Murashige and Skoog (MS) medium containing 3 mgL-1 Naphthalene Acetic Acid (NAA) when compared with MS medium containing combination of 3 mgL-1 NAA and 2 mgL-1 2,4 Dichlorophenoxyacetic acid (2,4-D). Induction of embryogenic calli from mature and immature leaf explants were evaluated on media supplemented with 3 mgL-1 NAA, 3 mgL-1 Indole-3-butyric acid (IBA) and 1 mgL-1 2,4-D. Immature leaf explants cultured on 1 mgL-1 2,4-D was found to be the most suitable for callusing.

Effect of 100 mgL-1 Casien hydrolysate, 0.1 mgL-1Abscisic acid (ABA), 0.5 mgL-1 6-Benzylaminopurine (BAP) and hormone-free MS media were tested for the maturation of calli and for the formation of somatic embryos. Casein hydrolysate was found to be the most suitable for maturation of calli. Matured embryogenic calli could be proliferated on MS medium supplemented with 0.5 mgL-1 BAP. Germination of embryos was evaluated in 0.1 mgL-1 BAP and 0.1 mgL-1 NAA combination, 0.02 mgL-1 NAA and 0.5 mgL-1 BAP as well as in hormone-free MS medium. The medium supplemented with 0.02 mgL-1 NAA and 0.5 mgL-1 BAP combination gave significantly higher germination. Further development of plantlets was achieved through germination of somatic embryos on hormone-free MS medium. Sixty percent of the plantlets could be successfully acclimatized on coir dust: sand=1:1 medium in a propagator.

Keywords: Leaf, hypocotyls, papaya, somatic embryogenesis, Carica papaya L. cv. Rathna, zygotic embryo.  

doi:10.4038/jnsfsr.v36i1.132

Journal of the National Science Foundation of Sri Lanka 36 (1) 41-50

How to Cite: Farzana, A., Palkadapala, P., Meddegoda, K., Samarajeewa, P. and Eeswara, J., 2008. Somatic embryogenesis in papaya (Carica papaya L.) cv. Rathna. Journal of the National Science Foundation of Sri Lanka, 36(1), pp.41–50. DOI: http://doi.org/10.4038/jnsfsr.v36i1.132
Published on 28 Mar 2008.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus