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Immunoglobulin classes of antibodies in the sera of buffaloes infected with Toxocara vitulorum

Authors:

S. T. Fernando ,

University of Peradeniya, Peradeniya, LK
About S. T.
Faculty of Veterinary Medicine & Animal Science
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Bimba T. Samarasinghe,

University of Peradeniya, Peradeniya, LK
About Bimba T.
Faculty of Veterinary Medicine & Animal Science
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Viranjani K. Gunawardena

University of Peradeniya, Peradeniya, LK
About Viranjani K.
Faculty of Veterinary Medicine & Animal Science
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Abstract

Serum samples of buffalo calves naturally infected with Toxocara vitulorum uere fractionated by means of 'Sephadex' G-200 gel-filtration. On gel-filtration, the buffalo sera separated into three main peaks sometimes with two minor peaks between peaks-1 and -2 as were those of other domestic animals. IgM and X2-macroglobulins were eluted mostly with the peak-1 and the trough following this peak. The IgG's were eluted largely with the peak-2 and the trough following this peak. On further fractionation the bulked and concentrated eluates of peak-2 resolved into two subclasses consisting of IgG2 and IgC1. The antibody activity of these classes was examined by the enzyme-liked immunosorbent, gel-diffusion, in vitro larval precipitin and passive cutaneous anaphylaxis techniques (ELISA, GPT, IVP, PCA). A phosphate buffered saline extract of infective T. vitulorum eggs was used as the antigen. The ELISA activity was distributed throughout the peaks-1 and -2, the trough between these peaks and that following peak-2. The GPT and IVP antibodies were present in the IgGl and not in IgG2 the immunoglobulin subclasses constituting the peak-2. The PCA activity was confined largely to the ascending part of peak-1 in the sera collected during the prepatent and patent phases of the infection whereas this activity was mostly confined to two minor peaks preceding peak-2 in the sera collected from days 174 of birth but after the experimental infection with 50,000-100,000 infective eggs on day 160.
How to Cite: Fernando, S.T., Samarasinghe, B.T. and Gunawardena, V.K., 1989. Immunoglobulin classes of antibodies in the sera of buffaloes infected with Toxocara vitulorum. Journal of the National Science Foundation of Sri Lanka, 17(2), pp.213–228. DOI: http://doi.org/10.4038/jnsfsr.v17i2.8221
Published on 27 Dec 1989.
Peer Reviewed

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