Start Submission Become a Reviewer

Reading: Characterization of the xylanolytic activity of Cellulomonas

Download

A- A+
Alt. Display

Research Articles

Characterization of the xylanolytic activity of Cellulomonas

Authors:

P. S. Peiris ,

University of New South Wales, P.O. Box 1, Kensington 2033, AU
About P. S.
School of Biotechnology
X close

Pamela A. D. Rickard,

University of New South Wales, P.O. Box 1, Kensington 2033, LK
About Pamela A. D.
School of Biotechnology
X close

Jan M. Daly

University of New South Wales, P.O. Box 1, Kensington 2033, LK
About Jan M.
School of Biotechnology
X close

Abstract

Maximum production of xylanolytic enzymes by Cellulomonas CSI-17 required aerobic conditions, a temperature of 300C and a pH between 6.2 and 7.2. Under these conditions, maximum levels of xylanolytic activity were reached within 48 hr; addition of further mineral salts and yeast extract to the growth medium decreased the time to 24 hr.

 

At pH values between 5.4 and 8.4 the xylanase was stable up to a temperature of 300C, for at least 48 hr after harvest; over the same temperature range, theb-xylosidase was stable only at the higher end of this pH range.

 

The culture filtrate was similar to the growing cells in its ability to hydrolyse xylan whilst the growing cells metabolized the bulk of the hydrolysis products, the latter accumulated as soluble reducing sugars when the culture filtrate was used as the enzyme source.

How to Cite: Peiris, P.S., Rickard, P.A.D. and Daly, J.M., 1983. Characterization of the xylanolytic activity of Cellulomonas. Journal of the National Science Foundation of Sri Lanka, 11(2), pp.293–306. DOI: http://doi.org/10.4038/jnsfsr.v11i2.8391
Published on 30 Jun 1983.
Peer Reviewed

Downloads

  • PDF (EN)

    comments powered by Disqus